Walkthrough on membrane models

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This walkthrough is a step-by-step guide that teaches how to extract subtomograms from structures with large surfaces (e.g., membranes) using the Dynamo surface models.

Data

We are going to demonstrate the basic ideas and tools on a synthetic dataset. This data is already available in the catalogue manager. It can be accessed by first opening the catalogue manager using the command:

dcm

After the catalogue manager opens, we create the synthetic tomogram that includes a large surface (membrane) in the following way:

Membrane step 1.png
Membrane step 2.png
Membrane step 3.png
Membrane step 4.png
Membrane step 5.png
Membrane step 6.png
Membrane step 7.png
Membrane step 8.png
Membrane step 9.png
Membrane step 10.png
Membrane step 11.png
Membrane step 12.png
Membrane step 13.png
Membrane step 14.png
Membrane step 16.png
Membrane step 17.png
Membrane step 18.png
Membrane step 19.png
Membrane step 20.png
Membrane step 21.png
Membrane step 22.png
Membrane step 23.png