Walkthrough on filament models
This walkthrough is a step-by-step guide that teaches how to extract subtomograms along filament-like structures using the Dynamo filament models.
We are going to demonstrate the basic ideas and tools on a synthetic dataset. This data is already available in the catalogue manager. It can be accessed by first opening the
>> dcm to start the catalogue manager:
We prepared a set of 6 tomograms, each containing one Immature HIV-1 virus like particle (VLP) with a layer formed by a lattice of capsid proteins. The capsid proteins have a C6 symmetry, a diameter of roughly 15nm and a molecular weight of about 150kDa. The pixelsize of the tomograms is 2.7 angstrom. You can find the tomograms in: