Difference between revisions of "Walkthrough on filament models"

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(Created page with "This walkthrough is a step-by-step guide that teaches how to extract subtomograms along filament-like structures using the ''Dynamo'' filament models. We are going to demons...")
 
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We prepared a set of 6 tomograms, each containing one Immature HIV-1 virus like particle (VLP) with a layer formed by a lattice of capsid proteins. The capsid proteins have a C6 symmetry, a diameter of roughly 15nm and a molecular weight of about 150kDa. The pixelsize of the tomograms is 2.7 angstrom. You can find the tomograms in:
 
We prepared a set of 6 tomograms, each containing one Immature HIV-1 virus like particle (VLP) with a layer formed by a lattice of capsid proteins. The capsid proteins have a C6 symmetry, a diameter of roughly 15nm and a molecular weight of about 150kDa. The pixelsize of the tomograms is 2.7 angstrom. You can find the tomograms in:
 
  <tt>~/data/tutorial_VLPs</tt>
 
  <tt>~/data/tutorial_VLPs</tt>
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Revision as of 12:01, 10 August 2021

This walkthrough is a step-by-step guide that teaches how to extract subtomograms along filament-like structures using the Dynamo filament models.


We are going to demonstrate the basic ideas and tools on a synthetic dataset. This data is already available in the catalogue manager. It can be accessed by first opening the


>> dcm to start the catalogue manager:


Data

We prepared a set of 6 tomograms, each containing one Immature HIV-1 virus like particle (VLP) with a layer formed by a lattice of capsid proteins. The capsid proteins have a C6 symmetry, a diameter of roughly 15nm and a molecular weight of about 150kDa. The pixelsize of the tomograms is 2.7 angstrom. You can find the tomograms in:

~/data/tutorial_VLPs

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