Walkthrough on creation of 3d scenes
This walkthrough shows how to create graphic depictions of tomogram contents. Those include:
- Slices of tomograms.
- Proteins located in the tomogram, with the orientations provided during the alignment.
- Geometrical objects (as membranes represented by triangulations).
The data set
This walkthrough works with the data set from the advanced starters guide. The goal is to depict:
- One slice of the tomogram in 3D.
This requires a tomogram file.
- The viruses placed an oriented along the directions found during a previous computed average.
This requires a template represe.
- A sketch of the membrane
This requires a model object.
From the Dynamo web
The tomogram can get fetched through:
in a linux shell, or through:
curl -O https://wiki.dynamo.biozentrum.unibas.ch/w/doc/data/fhv/crop.rec under MacOS.
The table, template and membrane model can be fetched similarly with names
https://wiki.dynamo.biozentrum.unibas.ch/w/doc/data/fhv/alignmentLocalRefinement.tbl https://wiki.dynamo.biozentrum.unibas.ch/w/doc/data/fhv/averagedLocalRefinement.em https://wiki.dynamo.biozentrum.unibas.ch/w/doc/data/fhv/membraneModel.omd
From your previos work
Assuming you have done the tutorial on the FHV data set, you can use the elements that you have already computed. Just extract them from the best project that you completed. Do not use a subboxing project: we want to get a template for the full crown. Examples below are given assuming that your best project was named localized, substitute if not.
Recover the last computed refined table in the data base of your project
ddb localized:rt -r t
and write it to a file
Fetch the last computed average
<tt>ddb localizedFullAngle:rt -r a
and write it into disk
If you created manually a membrane model, it should be inside your catalogue with the name mySurface (or any other you put). You can check inside the catalogue fhv
dcmodels fhv -nc mySurf
Here -nc mySurf will look for models with the string "mySurf" in the name. You should get a reply as:
Volume 1. Matching models: 1 (total: 1) /Users/casdanie/dynamo/examples/fhvParis/fhv/tomograms/volume_1/models/mySurface.omd
So you can read the model into a new variable (arbitrarily called m)
m = drread('Users/casdanie/dynamo/examples/fhvParis/fhv/tomograms/volume_1/models/mySurface.omd');
and write it to disk
Depicting a slice of a tomogram
The slice object
s = dpktomo.volume.slices.Slice();
Here s is just an object that will read data from a tomogram file to be defined, along with geometrical parameters as center, eulers (orientation), l, (sidelength)...
s.source = 'crop.rec'; s.center = 'center'; s.l = [1000,1000]; s.eulers = 'z'; s.fetchData();
The slice depictor object
We first create a figure with and empty axis </tt> f = figure; haxis = gca();hold on;
Here, f are handles that will be used later to direct the graphical output to our figure.
Now, we initialize a depiction object associated with the slice s sz = dpktomo.volume.slices.SliceGraphic(s); Note that no graphical output has been created yet. We first inform the object sz in which axis it is to be created.
sz.axis.h = haxis;
and then create the slice in the available axis.
If the figure is below other windows in your system you can rise it through
We can change the angle of view with the rotation icon or programmatically: view([-38,60]);