Programmatic reconstruction of tomograms

Dynamo includes several tools for creation of tomograms from initial tilt series. This articles presents an overview of the tools that can be used from the command line or inside scripts created by the users.

Overview

We assume that a raw tilt series raw.mrc is available, and also a set of markers that indexes the gold fiducials. Given this input, the creation of a tomogram will involve these steps.

• Creation of an aligned stack.
• Filtering of the aligned stack.
• Backprojection of the filtered aligned stack .

Weighting of aligned aligned stacks

The aligned stack cannot be directly backprojected. There are two ways to reweight its Fourier components so that aligned.mrc can be used to create

• Ramp filtering
• Sirt-like filer