Montage viewer for membrane picking

Here an example of use of the montage viewer for membrane models through the tomogram browser dtmslice is presented.

Starting the Montage Viewer

dtmslice view of a tomogram with several liposomes

The Montage viewer can be opened from the menu options in dtmslice

Opening the Montage viewer for the full scene

Options

With the default options, the montage shows at each z level the full projection of the intensity between a level and the next one.

Montage view of the full dtmslice scene

Model system

The model pool currently accessed by dtmslice is also available through the Montage Viewer. Let us define a membrane model.

Creation of a membrane model (called Surface in this menu)

Local zoom and drag

We make certain that the clicking option is active. This connects the tools for entering points into the model and to change the viewed scene in the montage (zoom and drag options).

The clicking status must be active in order to proceed with the clicking

With the full view, can inspect the scene and locate areas of interest.

We select one liposome for track across levels

After selecting the region of interest we can zoom on it, possibly also dragging the scene.

• Zoom in and out with the mouse wheel.
• Drag the scene by moving the mouse while the secondary button is pressed
  • While the scene is being dragged, the cursor should appear as a hand.

Remember than the clicking tool must be activated in otder to have these controls operative:

Zoom in a region of interest.

Adding points

After activating the clicker, you can (main) click on a z-level of the montage. Each click creates a point.

Membrane points clicked on the selected z level

The points created by clicking in the Montage Viewer will appear in dtmslice, and the other way round, as both viewers are connected to the same model pool.

The scene in dtmslice updates as we make annotations in the Montage Viewer

[Shift+C] creates a center point inside the membrane level

After visiting all the the zlevels where the membrane is defined, we can use the option for zooming out the view:

Icon in the toolbar for full zoom out

Full view

In this view, we can locate a new area of interest, create a new model and zoom again into the new area of interest