Difference between revisions of "Montage viewer for membrane picking"
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Here an example of use of the montage viewer for membrane models through the tomogram browser [[dtmslice]] is presented. | Here an example of use of the montage viewer for membrane models through the tomogram browser [[dtmslice]] is presented. | ||
+ | |||
+ | == Starting the Montage View === | ||
[[File:MontageMembranesTomogram.png|thumb|center|500px| [[dtmslice]] view of a tomogram with several liposomes ]] | [[File:MontageMembranesTomogram.png|thumb|center|500px| [[dtmslice]] view of a tomogram with several liposomes ]] | ||
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[[File:MontageMembranesMenu.png|thumb|center|500px| Opening the Montage viewer for the full scene]] | [[File:MontageMembranesMenu.png|thumb|center|500px| Opening the Montage viewer for the full scene]] | ||
+ | With the default options, the montage shows at each ''z'' level the full projection of the intensity between a level and the next one. | ||
[[File:MontageMembranesMontage.png|thumb|center|500px| Montage view of the full [[dtmslice]] scene ]] | [[File:MontageMembranesMontage.png|thumb|center|500px| Montage view of the full [[dtmslice]] scene ]] | ||
− | The [[model pool]] currently accessed by <tt>dtmslice</tt> is also available through the Montage Viewer. | + | The [[model pool]] currently accessed by <tt>dtmslice</tt> is also available through the Montage Viewer. Let us define a membrane model. |
[[File:MontageMembranesCreateModel.png|thumb|center|500px| Creation of a membrane model (called ''Surface'' in this menu) ]] | [[File:MontageMembranesCreateModel.png|thumb|center|500px| Creation of a membrane model (called ''Surface'' in this menu) ]] | ||
− | With can inspect the scene and locate areas of interest. | + | ==Local zoom and drag== |
+ | We make certain that the clicking option is active. This connects the tools for entering points into the model and to change the viewed scene in the montage (zoom and drag options). | ||
+ | [[File:MontageMembranesClicker.png|thumb|center|500px| The clicking status must be active in order to proceed with the clicking]] | ||
+ | |||
+ | With the full view, can inspect the scene and locate areas of interest. | ||
[[File:MontageMembranesLocateFirstGlobal.png|thumb|center|500px| We select one liposome for track across levels ]] | [[File:MontageMembranesLocateFirstGlobal.png|thumb|center|500px| We select one liposome for track across levels ]] | ||
+ | After selecting the region of interest we can zoom on it, possibly also dragging the scene. | ||
+ | * Zoom in and out with the mouse wheel. | ||
+ | * Drag the scene by moving the mouse while the secondary button is pressed | ||
+ | ** While the scene is being dragged, the cursor should appear as a hand. | ||
+ | Remember than the clicking tool must be activated in otder to have these controls operative: | ||
[[File:MontageMembranesLocateFirstLocal.png|thumb|center|500px| Zoom in a region of interest. ]] | [[File:MontageMembranesLocateFirstLocal.png|thumb|center|500px| Zoom in a region of interest. ]] | ||
− | |||
− | After activating the clicker, you can (main) click on a level of the montage. | + | == Adding points== |
+ | After activating the clicker, you can (main) click on a ''z''-level of the montage. Each click creates a point. | ||
[[File:MontageMembranesZoomedFirstSelection.png|thumb|center|500px| Membrane points clicked on the selected ''z'' level ]] | [[File:MontageMembranesZoomedFirstSelection.png|thumb|center|500px| Membrane points clicked on the selected ''z'' level ]] |
Revision as of 19:56, 27 February 2017
Here an example of use of the montage viewer for membrane models through the tomogram browser dtmslice is presented.
Starting the Montage View =
The Montage viewer can be opened from the menu options in dtmslice
With the default options, the montage shows at each z level the full projection of the intensity between a level and the next one.
The model pool currently accessed by dtmslice is also available through the Montage Viewer. Let us define a membrane model.
Local zoom and drag
We make certain that the clicking option is active. This connects the tools for entering points into the model and to change the viewed scene in the montage (zoom and drag options).
With the full view, can inspect the scene and locate areas of interest.
After selecting the region of interest we can zoom on it, possibly also dragging the scene.
- Zoom in and out with the mouse wheel.
- Drag the scene by moving the mouse while the secondary button is pressed
- While the scene is being dragged, the cursor should appear as a hand.
Remember than the clicking tool must be activated in otder to have these controls operative:
Adding points
After activating the clicker, you can (main) click on a z-level of the montage. Each click creates a point.
The points created by clicking in the Montage Viewer will appear in dtmslice, and the other way round, as both viewers are connected to the same model pool
After visiting all the the zlevels where the membrane is defined, we can use the option for zooming out the view:
In this view, we can localte a new area of interest, create a new model and zoom again into the area of interest