Getting a Structure from Multiple Tomograms of HIV Capsids

Introduction

The aim of this exercise is to get familiar with managing multiple tomograms through the catalogue and to develop strategies to reach a reasonable structure with a good choice of alignment projects. As this is an exercise and not a tutorial or walkthrough, only a few hints on how to proceed are given. The idea is to come up with your own solutions and to use the knowledge earned during the workshop in a realistic case that can still be solved within a reasonable amount of time. It is recommended to be familiar with at the advanced starters guide and the lattice walkthrough before starting this exercise.

Data

We prepared a set of 6 tomograms, each containing one VLP. (empiar id, paper)

Goal

Reach a resolution at around 10 angstrom, i.e., alpha helices should start to be visible in the final average. Hexamer.

Hints

Based on lattice walkthrough. Parameters. Use GPU on sciCORE.