Difference between revisions of "Getting a Structure from Multiple Tomograms of HIV Capsids"
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The aim of this exercise is to get familiar with managing multiple tomograms through the catalogue and to develop strategies to reach a reasonable structure with a good choice of alignment projects. As this is an exercise and not a tutorial or walkthrough, only a few hints on how to proceed are given. The idea is to come up with your own solutions and to use the knowledge earned during the workshop in a realistic case that can still be solved within a reasonable amount of time. It is recommended to be familiar with at the advanced starters guide and the lattice walkthrough before starting this exercise. | The aim of this exercise is to get familiar with managing multiple tomograms through the catalogue and to develop strategies to reach a reasonable structure with a good choice of alignment projects. As this is an exercise and not a tutorial or walkthrough, only a few hints on how to proceed are given. The idea is to come up with your own solutions and to use the knowledge earned during the workshop in a realistic case that can still be solved within a reasonable amount of time. It is recommended to be familiar with at the advanced starters guide and the lattice walkthrough before starting this exercise. | ||
Revision as of 18:32, 20 August 2018
The aim of this exercise is to get familiar with managing multiple tomograms through the catalogue and to develop strategies to reach a reasonable structure with a good choice of alignment projects. As this is an exercise and not a tutorial or walkthrough, only a few hints on how to proceed are given. The idea is to come up with your own solutions and to use the knowledge earned during the workshop in a realistic case that can still be solved within a reasonable amount of time. It is recommended to be familiar with at the advanced starters guide and the lattice walkthrough before starting this exercise.
Data
We prepared a set of 6 tomograms, each containing one VLP. (empiar id, paper)
Goal
Reach a resolution at around 10 angstrom, i.e., alpha helices should start to be visible in the final average. Hexamer.
Hints
Based on lattice walkthrough. Parameters. Use GPU on sciCORE.