Difference between revisions of "Getting a Structure from Multiple Tomograms of HIV Capsids"

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== Introduction ==
 
 
The aim of this exercise is to get familiar with managing multiple tomograms through the catalogue and to develop strategies to reach a reasonable structure with a good choice of alignment projects. As this is an exercise and not a tutorial or walkthrough, only a few hints on how to proceed are given. The idea is to come up with your own solutions and to use the knowledge earned during the workshop in a realistic case that can still be solved within a reasonable amount of time. It is recommended to be familiar with at the advanced starters guide and the lattice walkthrough before starting this exercise.
 
The aim of this exercise is to get familiar with managing multiple tomograms through the catalogue and to develop strategies to reach a reasonable structure with a good choice of alignment projects. As this is an exercise and not a tutorial or walkthrough, only a few hints on how to proceed are given. The idea is to come up with your own solutions and to use the knowledge earned during the workshop in a realistic case that can still be solved within a reasonable amount of time. It is recommended to be familiar with at the advanced starters guide and the lattice walkthrough before starting this exercise.
  

Revision as of 18:32, 20 August 2018

The aim of this exercise is to get familiar with managing multiple tomograms through the catalogue and to develop strategies to reach a reasonable structure with a good choice of alignment projects. As this is an exercise and not a tutorial or walkthrough, only a few hints on how to proceed are given. The idea is to come up with your own solutions and to use the knowledge earned during the workshop in a realistic case that can still be solved within a reasonable amount of time. It is recommended to be familiar with at the advanced starters guide and the lattice walkthrough before starting this exercise.

Data

We prepared a set of 6 tomograms, each containing one VLP. (empiar id, paper)

Goal

Reach a resolution at around 10 angstrom, i.e., alpha helices should start to be visible in the final average. Hexamer.

Hints

Based on lattice walkthrough. Parameters. Use GPU on sciCORE.