Difference between revisions of "Fourier Shell Correlation"

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With this definition, the first element in a FSC would measure the similarity of A and B restricted to the first shell A<sub>1</sub> and B<sub>1</sub>. This shell is just the single central Fourier pixel in both cases: the zero-th component of the Fourier transform. In the other extreme of resolution, FSC(N/2) would be measuring the similarity of those Fourier modes in A and B that are ''strictly worse'' that the resolution 1/(N/2), but ''better'' than the resolution 1/(N/2-1).
 
With this definition, the first element in a FSC would measure the similarity of A and B restricted to the first shell A<sub>1</sub> and B<sub>1</sub>. This shell is just the single central Fourier pixel in both cases: the zero-th component of the Fourier transform. In the other extreme of resolution, FSC(N/2) would be measuring the similarity of those Fourier modes in A and B that are ''strictly worse'' that the resolution 1/(N/2), but ''better'' than the resolution 1/(N/2-1).
  
Don't get confused about the notation. FSC(i)|<sub>i=1</sub> corresponding to the Fourier component of order zero (and not one) just means that we start counting the components of the FSC vector with index i=1 (up to i=N/2). The first fourier component A(0) corresponds to
+
Don't get confused about the notation. FSC(i)|<sub>i=1</sub> corresponding to the Fourier component of order zero (and not one) just means that we start counting the components of the FSC vector with index i=1 (up to i=N/2). The first fourier component A(0) has fixed definition, and it corresponds to
 
<tt>
 
<tt>
                    N
+
            N
      A(k) =     sum  a(n)*exp(-j*2*pi*(k-1)*(n-1)/N), 1 <= k <= N.
+
A(k) = sum  a(n)*exp(-j*2*pi*(k-1)*(n-1)/N), 1 <= k <= N.
                    n=1
+
            n=1
 
</tt>
 
</tt>
for k = 0;
+
for k = 0 (i.e, the total intensity of particle ''a'' in Fourier space).

Revision as of 10:34, 25 April 2016

The Fourier Shell Correlation (FSC) between two volumes is a measure of their similarity. It assigns a coefficient between -1 and 1 to each chosen frequency range.

FSC computations are used in different areas of Dynamo

  1. During adaptive bandpassing, the FSC of the two half averages is used to measure the resolution attained at each iteration.
  2. During a regular project, the FSC between the freshly computed iteration average and the one generated in the previous iteration is also computed. This information is used to measure the convergence.


The command line that computes the FSC is dfsc. In its default settings, the FSC for two volumes with a sidelength of N pixels would be a vector with a length of "N"/2 pixels.

  1. compute A as fourier transform of real space volume a
  2. compute B as fourier transform of real space volume a
  1. for each shell index i=1:N/2
    compute a discretized shell n(i) as a volume with entries 0 or 1
    a fourier pixel with a distance k from the center is in shell n(i) if i-1<=|k|<i
    locate elements of A and B inside shell n(i)
    Ai = A|n(i) ; a vector with as many entries as non-zero elements in n(i)
    Bi = B|n(i) ; a vector with as many entries as non-zero elements in n(i)
    compute cross correlation of vectors Ai, Bi:
    FSC(i) = Ai * Bi / |Ai| |Bi|
    * represents pixelwise multiplication, |.| is the vector norm.

With this definition, the first element in a FSC would measure the similarity of A and B restricted to the first shell A1 and B1. This shell is just the single central Fourier pixel in both cases: the zero-th component of the Fourier transform. In the other extreme of resolution, FSC(N/2) would be measuring the similarity of those Fourier modes in A and B that are strictly worse that the resolution 1/(N/2), but better than the resolution 1/(N/2-1).

Don't get confused about the notation. FSC(i)|i=1 corresponding to the Fourier component of order zero (and not one) just means that we start counting the components of the FSC vector with index i=1 (up to i=N/2). The first fourier component A(0) has fixed definition, and it corresponds to

            N

A(k) = sum a(n)*exp(-j*2*pi*(k-1)*(n-1)/N), 1 <= k <= N.

           n=1

for k = 0 (i.e, the total intensity of particle a in Fourier space).