Filament model

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Filament models are used for geometries where location and orientation of particles to be cropped (called subunitsin this context) can be defined with relation to tubular estructures.

The usual way of handling them is by opening a small fraction of a tomogram inside dtmslice and mark manually positions that will be used to define a "backbone". Loosely speaking, we call the "backbone of the filament" to the series of ordered points that arise from defining successive orthogonal sections along the path of the filament and picking the central point. Different models are available for the different relationships between the "backbone" of the filament and the locations and positions of the particles to be cropped.


Input stage

The input points in a filament model are used to define a smooth interpolant called backbone.

Clicking input points in dtmslice

Computation stage

Different models are provided to account for different ways of relating particles to the backbone.

filamentWithTorsion

The subunits will be defined along the backbone. The center of each subunit will be defined in the backbone, and Parameters:

  • dz distance of the subunits along the backbone (in pixels)
  • dphi angular distance between the subunits (in degrees).
* fdf

filamentSubunitsObHelix

Parameters:

  • dz distance of the subunits along the backbone (in pixels)
  • dphi angular distance between the subunits (in degrees)
  • radius distance of the subunits to the backbone (in pixels)