Averaging of subtomograms

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Category:General steps

Averaging of subtomograms means applying an available set of alignment parameters onto a set of data particles and adding them together. Because of the missing wedge, a Template:Fourier compensation during averaging in the Fourier space is operated during this step.

From the operative point of view, the particles are stored in a data folder, which are indexed by a table.



Through the command line

To operate manually averages of particles (given an available Template:Table), use the daverage command, or its GUI version {{t|dvarage_GUI}. daverage


Inside an alignment project

At the end of each iteration, the refined table generated by Dynamo is used to produce an average, which will be used as starting template in the next iteration.

There are different project parameters that can be used to modify the default behaviour of the daverage command used inside an alignment project.