Difference between revisions of "Advanced starters guide"

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Revision as of 18:51, 15 July 2017

This walkthrough uses a small size example based on a real tomogram to covers several tasks.


The example data set

The data is a fraction of a tomogram. The full tomogram was used in "Cryo-electron tomography reveals novel features of a viral RNA replication compartment." (Ertel et al.), and represents several FHV viruses docked in the membrane of a mythocondrion.


Downloading

In principle, you can download all the files related to this example with the command:

 dpkhelp.wiki.downloadExample('fhv');

If it fails under Matlab or the Dynamo command line, you can try to directly use the linux order

wget  https://wiki.dynamo.biozentrum.unibas.ch/w/doc/data/fhv/crop.rec 

or

curl -O  https://wiki.dynamo.biozentrum.unibas.ch/w/doc/data/fhv/crop.rec 

unter MacOS.

This should have created the file called crop.rec in your current directory. You're probably curious to see what's inside, so that let's write first:

dfile crop.rec

to let Dynamo check the dimensions of the file. The header of a .rec file is readed as a regular mrc, yielding:

 
 filetype: volume
 size: 1285 x 956 x 786