EMBO workshop 2021
Revision as of 10:31, 24 June 2021 by Daniel Castaño (talk | contribs)
These tutorials takes place virtually on the 11th and 13th of September of 2021 (14-18:30), as a part of the EMBO workshop for Image processing in cryoEM
Contents
Tutorials
Connection
Please refer to your course material to access your credentials.
Dynamic of the course
The course is divided on different modules, described in the time schedule below. Each module is structured in three stages:
- Short description, demostration.
- Demostration
- definition 1
- Individual work
- definition 2-1
- definition 2-2
- Common work
Time schedule
Day 1: tomography
| day | 'time |
| 14:00 | Introduction |
| 14:30 | tilt series alignment: manual |
| 15:00 | tilt series alignment (GUI) |
| 15:30 | tilt series alignment (command line) |
| 16:00 | Break |
| 16:30 | models: catalogue system |
| 17:00 | models: helical symmetry |
| 17:30 | models: vesicles and surfaces |
| 18:00 | Template matching |
Day 2: Subtomogram averaging
| 'time | subject |
| 14:00 | Introduction |
| 14:30 | Getting started: synthetic thermosomes |
| 15:30 | Advanced tutorial: FHV |
| 16:00 | Break |
| 16:30 | FHV: continuation |
| 17:00 | Seed oversampling |
| 18:00 | High resolution pipelines |
Contents
Alignment of tilt series
Data location: t.b.d.
Manual alignment of tilt series
We will click manually on the gold beads of our test tilt series
Automated alignment of tilt series through the GUI
Automated alignment of tilt series through the command line
Models
Catalogues
Helical models
Vesicle and surface models
Reusing models
Template matching
Subtomogram averaging
Introduction
Advanced starters guide
Seed oversampling
High resolution pipeline
Not a tutorial per se: this is a presentation on a set of tools to interconnect Dynamo, M and other software.
