Programmatic reconstruction of tomograms
Revision as of 17:34, 27 June 2018 by Daniel Castaño (talk | contribs) (Created page with "''Dynamo'' includes several tools for creation of tomograms from initial tilt series. This articles presents an overview of the tools that can be used from the command line or...")
Dynamo includes several tools for creation of tomograms from initial tilt series. This articles presents an overview of the tools that can be used from the command line or inside scripts created by the users.
Contents
Overview
We assume that a raw tilt series raw.mrc is available, and also a set of markers that indexes the gold fiducials. Given this input, the creation of a tomogram will involve these steps.
- Creation of an aligned stack.
- Filtering of the aligned stack.
- Backprojection of the filtered aligned stack .
Creation of aligned stacks
Creation of an aligner object
Applying the an aligner object
Use of the alignment object
Weighting of aligned aligned stacks
The aligned stack cannot be directly backprojected. There are two ways to reweight its Fourier components so that aligned.mrc can be used to create
- Ramp filtering
- Sirt-like filer
