Utilities for stack creation with the Hagen scheme
Here we describe some parsers we frequently use to organize the data folders delivered by the microscope operators into single stack files. This utility just:
- parses the structure of the input folder
- creates a correctly formatted input for the Imod command alignframes
- creates the corresponding Imod order and
- submits it to Imod
Thus, you'll need to have Imod activated in your session to use this parser
Contents
Input raw data folder
A typical input raw data folder contains
- a gain reference file (with extension .dm4)
- a defect file (with extension .txt)
- a series of files, each one for each tilt angle.
CountRef_FtsH_23_000_-0.0_Sep07.dm4 defects_FtsH_000_0.0_Sep06.txt FtsH_23_000_-0.0_Sep07.mrc FtsH_23_001_3.0_Sep07.mrc FtsH_23_002_-3.0_Sep07.mrc . . . . FtsH_23_027_-42.0_Sep07.mrc FtsH_23_028_42.0_Sep07.mrc FtsH_23_029_45.0_Sep07.mrc FtsH_23_030_-45.0_Sep07.mrc
Imod wrappers
Creates the wrapper object
p = <full path to your directory>; a = dpcomp.hagen.StackConverter(); a.parseSourcePath(p);
It will detect the contents of the file, and check that it can understand the ordering of the micrographs.
Order the projection files
You can display if the files where correctly parsed:
a.listFiles();
and, if so, you can then create a text file for them
a.writeList();
which will be called by default orderedFiles.list and will be located on the same directory. You can also create text file for the tilt angles with a.writeTiltList(); This will create by default the file stack.tlt
Align the frames
Imod order
The created order for imod will have the structure
alignframes -gain GAINFILE -defect DEFECTFILE -mode 2 -list LIST STACK
By default, the target STACK will be called stack.mcr.
To create and run it, type:
a.imodAlignFrames();
If the target STACK already exist in disk, Dynamo will show the formatted order, but will abort before submitting it to Imod.
Troubleshouting
You need to activate Imod before opening Matlab (or before starting the Dynamo standalone session).
