Particle extraction
Particle extraction is the process of using a set of positions (and possibly orientations) defined on a set of tomograms to create a set of subtomograms files. This set is represented by a data folder containing one file for each cropped subtomogram, and possibly a table retaining the metadata. Data folder and table can directly be used for subtomogram alignment
Contents
Using the catalogue
Select the tomograms in which you have models containing particles and click on the menu tab Crop particles. A new GUI will popup, with a list of models. You can then select the models that already contain crop points). Then click on Create list. This will create a text file with extension .vll just for information. Click then on -> Crop particles, getting a GUI where you can select the sidelength in pixels, and start the actual cropping.
From the command line
You might just use the command dtcrop. Help on command: dtcrop
On a single tomogram
On several tomograms tomogram
On each model separately
When you are manually creating a model in one of the browsers, the real workflow in an actual project involving many tomograms and modeles would be to just click the necessary points to define the user input of the model, store it in the catalogue, and move to the next model.
