Difference between revisions of "Pasteur workshop 2017"

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== Program==
 
== Program==
  
=== Introduction to tomography and subtomogram averaging ===
+
===Introduction to tomography and subtomogram averaging===
 
   45 minutes of presentation to introduce tomography, subtomogram averaging and ''Dynamo''.
 
   45 minutes of presentation to introduce tomography, subtomogram averaging and ''Dynamo''.
  
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on screen presentation/hands-on practical. We will follow together the [[starters guide | basic hands-on tutorial]] of ''Dynamo''.
 
on screen presentation/hands-on practical. We will follow together the [[starters guide | basic hands-on tutorial]] of ''Dynamo''.
 
  Advanced getting started.
 
  Advanced getting started.
 +
 
=== Advanced getting started===
 
=== Advanced getting started===
 
  After the coffee break at 16:00 participants will follow the [[advanced starters guide| advanced introduction]] on a reduced data set from a real tomogram.
 
  After the coffee break at 16:00 participants will follow the [[advanced starters guide| advanced introduction]] on a reduced data set from a real tomogram.

Revision as of 19:00, 18 July 2017

A guide to use Dynamo during the practical session of the Pasteur course INTEGRATIVE STRUCTURAL BIOLOGY​ - Mechanisms of Living Organisms, in the afternoon session of the 19th of July of 2017.

Computer access

Access the course account with your password.

Opening a Linux terminal

We need to create a command terminal. Right click on the screen and select Open Terminal in the menu . A Linux command window will popup. Please create a folder for this course.

mkdir tomo
 cd  tomo 

Starting Dynamo

In the terminal open Matlab through:

matlab & 
(it will take some secons). Then, in the Matlab window write:
 run /ISB2017/dynamo/dynamo_activate.m 

This will activate Dynamo in the current Matlab session.

Data

The tomogram for the second tutorial is located in the location /ISB2017/Course_material/July19/crop.rec

Make a local copy by typing in the Matlab window:

!cp /ISB2017/Course_material/July19/crop.rec . 

(the "!" signs just passes the cp order to Linux)


Program

Introduction to tomography and subtomogram averaging

 45 minutes of presentation to introduce tomography, subtomogram averaging and Dynamo.

Tutorial 1: Getting started

on screen presentation/hands-on practical. We will follow together the basic hands-on tutorial of Dynamo.

Advanced getting started.

Advanced getting started

After the coffee break at 16:00 participants will follow the  advanced introduction on a reduced data set from a real tomogram.

Instructors

  1. Daniel Castaño-Díez, BioEM Lab, University of Basel
  2. Paula Pérez-Navarro, C-CINA, University of Basel
  3. Cynthia Taveneau, Institut Curie, Paris