Difference between revisions of "Cross correlation matrix"
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This similarity of particles ''i'' and ''j'' measured in terms of the normalized cross correlation of the the two aligned particles, filtered to their common fourier components, and restricted to a region in direct space (indicated by a classification mask). The pseudo code will run as: | This similarity of particles ''i'' and ''j'' measured in terms of the normalized cross correlation of the the two aligned particles, filtered to their common fourier components, and restricted to a region in direct space (indicated by a classification mask). The pseudo code will run as: | ||
<code> | <code> | ||
| + | # Input | ||
| + | #: particles p<sub>i</sub> and p<sub>j</sub> | ||
| + | #: alignment parameters A<sub>i</sub> and A<sub>j</sub> | ||
| + | #: classification mask m | ||
# read particles ''i'' and ''j'' -> p<sub>i</sub>, p<sub>j</sub> | # read particles ''i'' and ''j'' -> p<sub>i</sub>, p<sub>j</sub> | ||
# align particles ''i'', and ''j'' -> A<sub>i</sub>p<sub>i</sub>, A<sub>j</sub>p<sub>j</sub> | # align particles ''i'', and ''j'' -> A<sub>i</sub>p<sub>i</sub>, A<sub>j</sub>p<sub>j</sub> | ||
| Line 16: | Line 20: | ||
#: p<sub>i</sub> = F<sup>-1</sup>[ F(p<sub>i</sub>).* C<sub>ij</sub> ] | #: p<sub>i</sub> = F<sup>-1</sup>[ F(p<sub>i</sub>).* C<sub>ij</sub> ] | ||
# compare the filtered particles inside the classification mask. | # compare the filtered particles inside the classification mask. | ||
| + | #: ccmatrix(i,j)<- normalized cross correlation | ||
</code> | </code> | ||
| + | &ptilde | ||
==Input for ccmatrix construction== | ==Input for ccmatrix construction== | ||
Revision as of 10:54, 19 April 2016
The cross correlation matrix (often called ccmatrix in Dynamo jargon) of a set of N particles is an N X N matrix. Each entry (i,j) represents the similarity of particles i and j in the data set.
Contents
Definition of similarity
This similarity of particles i and j measured in terms of the normalized cross correlation of the the two aligned particles, filtered to their common fourier components, and restricted to a region in direct space (indicated by a classification mask). The pseudo code will run as:
- Input
- particles pi and pj
- alignment parameters Ai and Aj
- classification mask m
- read particles i and j -> pi, pj
- align particles i, and j -> Aipi, Ajpj
- compute missing wedges for particles i and j -> Wi, Wj
- rotate missing wedges -> RiWi, RjWj
- compute Fourier coefficients common to bCoth rotated missing wedges
- Cij = intersection(RiWi, RjWj)
- filter pi with Cij
- pi = F-1[ F(pi).* Cij ]
- compare the filtered particles inside the classification mask.
- ccmatrix(i,j)<- normalized cross correlation
&ptilde
Input for ccmatrix construction
The minimal input is a set of particles (formatted as a data folder) and their alignment parameters (formatted as a table). You also may want to specify a classification mask and optatively additional mathematical operations on the particles (as bandpassing or symmetrization).
Computation of a ccmatrix
Dynamo typically implements the computation of a ccmatrix in independent blocks that can be handled separately by different cores.
Application of a ccmatrix
A ccmatrix is most commonly used in the context of PCA classification
